Xref: utzoo sci.bio:2107 sci.med:10967
Path: utzoo!utgpu!jarvis.csri.toronto.edu!rutgers!usc!apple!ames!hc!lanl!beta!dd
From: dd@beta.lanl.gov (Dan Davison)
Newsgroups: sci.bio,sci.med
Subject: references on multiple sequencing to reduce the error rate
Keywords: Maxam-Gilbert, Sanger, nucleic acid, sequencing
Message-ID: <27540@beta.lanl.gov>
Date: 3 Jul 89 18:05:56 GMT
Organization: Los Alamos National Laboratory
Lines: 24


I need a reference to any study of how many times one needs to sequence
a given template to reduce (or eliminate) the error level as much as possible.

When sequencing first came out in preprint form ('76, '77 or
thereabouts) I do recall this subject being discussed a great deal.  I
just don't remember any publications on the subject now.  

The general idea is that even if your gels are clean, banding perfect,
and all those other unlikely things, the chemical and dideoxy methods
will have a certain error rate.  This is generally, but not entirely,
due to misincorporation of the ddNTPs by the Klenow or other polymerase
(for the dideoxy method) or random non-specific cleavage (chemical
method).  

The usual thing is to sequence both strands and pray, but I'm interested
in studies of the problem.

TIA,
dan davison/theoretical biology/t-10 ms k710/los alamos national laboratory
los alamos, nm 875545/dd@lanl.gov (arpa)/dd@lanl.uucp(new)/..cmcl2!lanl!dd
-- 
dan davison/theoretical biology/t-10 ms k710/los alamos national laboratory
los alamos, nm 875545/dd@lanl.gov (arpa)/dd@lanl.uucp(new)/..cmcl2!lanl!dd