Path: utzoo!utgpu!watmath!uunet!bionet!bloom-beacon!athena.mit.edu!steve From: steve@athena.mit.edu (Stephen S. Gisselbrecht) Newsgroups: bionet.molbio.methds-reagnts Subject: Looking for a protocol... Keywords: erythrocyte loading, sucrose gradient Message-ID: <12501@bloom-beacon.MIT.EDU> Date: 8 Jul 89 01:53:34 GMT Sender: daemon@bloom-beacon.MIT.EDU Reply-To: steve@athena.mit.edu (Stephen S. Gisselbrecht) Organization: Massachusetts Institute of Technology Lines: 13 Hi. I am trying to load macromolecules into erythrocyte ghosts. Does anyone have a protocol for separating loaded from unloaded cells through a sucrose gradient? Please e-mail responses. (INTERNET: steve@athena.mit.edu USENET: mit-eddie!steve) Thank you. steve