Path: utzoo!utgpu!watmath!uunet!bionet!mrc-crc.ac.uk!mfarrall
From: mfarrall@mrc-crc.ac.uk (Martin Farrall)
Newsgroups: bionet.jobs
Subject: (none)
Message-ID: <8908040841.AA07687@net.bio.net>
Date: 4 Aug 89 08:32:32 GMT
Sender: daemon@NET.BIO.NET
Lines: 43

POSTDOCTORAL POSITION IN MAMMALIAN DEVELOPMENTAL MOLECULAR GENETICS

      Most developmental malformations in humans have complex
aetiologies that are poorly understood.  However, several
rare 'single gene' syndromes have been identified which are
amenable to 'reverse genetic' strategies.  A phenotypically
similar developmental mutation in humans and mice that maps
to homologous chromosomal segments has been described, Greig
cephalopolysyndactyly syndrome (GCPS, human chromosome 7p13)
and extra-toes (Xt, proximal mouse chromosome 13).  GCPS has
been localized by cytogenetic analysis of translocation
breakpoints and linkage analysis, Xt has been mapped by
linkage to proximal chromosome 13 markers.  We propose to
localize the GCPS translocation breakpoints within a long
range restriction map, chromosome walk and/or jump towards
the breakpoint and identify potential coding sequences.
Progress towards this goal will be monitored by genetic
mapping of conserved sequences in mice using interspecific
crosses segregating for Xt.  Candidate genes will be cloned
for expression and sequencing studies.  Subsequent gene
analysis (in situ expression studies and transgenic mice to
confirm the identity of 'candidate' genes) will dissect the
molecular basis of these malformation syndromes.

      I have recently received funding from the Medical
Research Council (UK) to commence this 'reverse genetic'
project aimed at cloning the gene(s) mutated in GCPS and Xt
(over 5 years).  I am looking for a postdoctoral research
assistant to make a committed contribution to this project, a
training in molecular genetics is essential.  Work will
include 'HTF island' cosmid library construction, pulse field
mapping and cDNA library construction and screening.


	If you are interested in further details please contact:

Martin Farrall, Division of Molecular Medicine, Clinical
Research Center, Northwick Park Hospital, Watford Road,
Harrow, Middlesex, HA1 3UJ, UK.

Telephone: +44 1 422 1442        FAX: +44 1 423 1275

E-mail: mfarrall%uk.ac.crc@ac.uk  JANET/EARN/BITNET