Path: utzoo!attcan!uunet!bionet!brutlag
From: brutlag@NET.BIO.NET (Doug Brutlag)
Newsgroups: bionet.general
Subject: Re: cloning repeated sequences
Message-ID: <Aug.24.16.13.10.1989.10310@NET.BIO.NET>
Date: 24 Aug 89 23:13:11 GMT
References: <8908242018.AA04264@net.bio.net>
Organization: Natl Computer Resource for Mol. Biology
Lines: 22

Mike,

	You might use the strains that we used to clone Drosophila
satellite DNAs.  We found that 60 to 100 copies of repeats 5 to 15
base pairs long were stable.  The best references are:

Lohe, A. R., & Brutlag, D. L. (1986). Multiplicity of satellite DNA
sequences in Drosophila melanogaster.. Proc Natl Acad Sci U S A,
83(3), 696-700.

Lohe, A. R., & Brutlag, D. L. (1987). Adjacent satellite DNA segments
in Drosoph
Lohe, A. R., & Brutlag, D. L. (1987). Adjacent satellite DNA segments
in Drosophila:: structure of junctions.. J Mol Biol, 194(2), 171-9.

Lohe, A. R., & Brutlag, D. L. (1987). Identical satellite DNA
sequences in sibling species of Drosophila. J Mol Biol, 194(2),
161-70.

	However, your instability may also be sequence dependent.

Doug Brutlag