Path: utzoo!utgpu!watmath!uunet!shelby!agate!codon1.berkeley.edu!ladasky
From: ladasky@codon1.berkeley.edu (John Ladasky;1021 Solano No. 2;528-8666)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Introduction of Liposomes to Cells
Message-ID: <1989Sep1.215356.11571@agate.uucp>
Date: 1 Sep 89 21:53:56 GMT
References: <8908311640.AA10975@net.bio.net>
Sender: usenet@agate.uucp (USENET Administrator;;;;ZU44)
Reply-To: ladasky@codon1.berkeley.edu.UUCP (John Ladasky)
Organization: University of California, Berkeley
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	Flavio Codeco was looking for a good liposome preparation technique.
I would also be interested in this, although I'm looking for larger vesicles -
say 100-200 nm.

	Also, I would be most interested in hearing about any non-destructive
methods for fusing such liposomes with human cells, specifically leucocytes.
Folks where I work are having trouble introducing liposomes to cells without
thrashing the cytoplasm.  We're working on a reagent-delivery mechanism for a
flow cytometry assay, so the protocol needn't be anything for in vivo use...

	Thanks in advance.

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_______________________________________________________________________________
"Do unto others as you would like		       - John J. Ladasky ("ii")
 to do unto them. " Richard Bach		  (ladasky@enzyme.berkeley.edu)