Path: utzoo!attcan!uunet!bionet!bmc1!msz From: MSZ@bmc.uu.se (Michael Szardenings) Newsgroups: bionet.molbio.methds-reagnts Subject: Message-ID: <2430@bmc.uu.se> Date: 6 Sep 89 08:47:54 GMT References: <12522823357.23.RLUDWIG@BIONET-20.BIO.NET> Organization: Biomedical Center, University of Uppsala, Sweden Lines: 18 In article <12522823357.23.RLUDWIG@BIONET-20.BIO.NET>, RLUDWIG@BIONET-20.BIO.NET (Robert Ludwig) writes: > We and others are having problems with Boehringer Mannheim calf intestine > phosphatase (CIP), until recently the best available. Activities of > recently purchased enzyme are low and yield only incomplete digests. Boehringer had some problems with the enzyme in the last 1-2 years. As they had delivery problems (probably they changed the source or the purification) me and other people of whom I know, that you can trust them, switched to Pharmacia's CIP. But take care, what you will do with your DNA, some prepara- tions are available in Ammoniumsulfate ! Nevertheless, I never heard anybody complaining about low activities. Michael Szardenings Internet : MSZ@BMC.UU.SE University of Uppsala Bitnet : MSZ@SEMAX51 Biomedical Center Dep. Mol. Biol. Box 590 751 24 Uppsala Sweden (----> that does not mean I'm paid by Pharmacia !)