Path: utzoo!utgpu!watmath!iuvax!genet!bionet!templevm.bitnet!V5222E
From: V5222E@templevm.bitnet (Win)
Newsgroups: bionet.molbio.evolution
Subject: Re: systematics conf
Message-ID: <8911110249.AA14930@net.bio.net>
Date: 11 Nov 89 01:30:57 GMT
References: <dbd%benden@LANL.GOV>
Sender: daemon@NET.BIO.NET
Lines: 27

Trees, genes and Cucumbers....

Time to put in a few questions to the computing/molevol community.
Here is a problem that I am just starting to tackle, and welcome  input on:
I am writing a grant for a (very loosley defined as yet) a question on
Holothuria (them sea Cukes). Seems that Cukes have had a bad wrap when it comes
 to previous molecular trees... or at least a somewhat inaccurate one.
Also, not many have had their DNA sequenced for intersting Phylo Chr$.
I want to tackle a Cuke tree, with several ideas in mind.
1) Some of the species are as yet undescribed or even Named
2) I want to be very careful in my choice of gene, as I have seen serious
   problems with Ribosomal resolution cropping up amongst Echinoderms in gerner
al and don't want to get embroiled in a messy story. I am not throwing out
Ribo sequencing tho, as I feel there is good data there if enough Chr$ are
available.
3) I wonder at the variablity amongst these orgs. If I were looking at
  Inter-specific relationships, as opposed to inter class, could I use
  Mitochondrial gene instead? I worry about interspecific CF with Ribos.
4) How about RESACT? This intersting molecule that Urchins use as an attractant
  for sperm. Anyone heard of similar gene coded molecules in other echinoderms?
 It would be a very interesting gene to look at, but not much known.
This is all basically just free association for now. Anyone got anything
interesting to say?
(You too Dan)

Thanks for your indulgence.
Win Hide.