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From: davison@UHNIX2.UH.EDU (Dan Davison)
Newsgroups: bionet.molbio.evolution
Subject: Re: systematics conf
Message-ID: <8911120253.AA19350@net.bio.net>
Date: 12 Nov 89 02:52:49 GMT
References: <8911110249.AA14930@net.bio.net>
Sender: daemon@NET.BIO.NET
Lines: 35

> I want to tackle a Cuke tree, with several ideas in mind.

Be kind to them, they're in a strange place ;->>





> 2) I want to be very careful in my choice of gene, as I have seen serious
> problems with Ribosomal resolution cropping up amongst Echinoderms in gerner
> al and don't want to get embroiled in a messy story. [...]

If one is careful, there is no problem.  You are right to be
suspicious;  however, until you sequence you cannot tell whether the
rRNA is inappropriate (a la Giardia vs Varicela nextrix (sp mangled,
sorry).

> 3) I wonder at the variablity amongst these orgs. If I were looking at
>   Inter-specific relationships, as opposed to inter class, could I use
>   Mitochondrial gene instead? I worry about interspecific CF with Ribos.

Probably.  You could make a fast check with 4-cutter restriction
enzymes on mt DNA.  What's "CF"?  I assume it's not cystic fibrosis.

> (You too Dan)

Gosh gee thanks.


-- 
dr. dan davison/dept. of biochemical and biophysical sciences/univ. of
Houston/4800 Calhoun/Houston,TX 77054-5500/davison@uh.edu/DAVISON@UHOU

Disclaimer: As always, I speak only for myself, and, usually, only to
myself.