Path: utzoo!attcan!uunet!samsung!brutus.cs.uiuc.edu!apple!genbank!sysh.surrey.ac.uk!MBY134
From: MBY134@sysh.surrey.ac.uk
Newsgroups: bionet.molbio.evolution
Subject: (none)
Message-ID: <8911241751.AA14984@net.bio.net>
Date: 24 Nov 89 15:13:04 GMT
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                           RFLP SEARCH

     A while ago I sent a message to the bulletin board asking if
anyone had any programs that could be used to store, match and
compare (given gel reading errors) RFLP profiles. I had a few
useful replies and a lot of requests from others interested in
any answers I received. I thought therefore that I would post
what I've got so far.

Firstly, J. Rogers, University of Utah [ROGERS@EDU.UTAH.ARSUN],
posted me a program he coded from one of Nei's logarithms for
dealing with restriction enzyme data. This is primarily to use
estimates of level of base substitution, between DNA samples,
obtained from RE data to construct phylogenies. Although this is
useful to me for other purposes, it was not exactly what I had in
mind.

Ambis Systems, Inc, 33939 Ruffin Rd., San Diego, CA 92123
[VISLA@EARN.UMDC], posted me details of a "Pattern matching -
Microbiology" program, constructed for their AMBIS scanner for
automatic reading of gels and autorads, that sounds like it may
be just what I had in mind. They said that it would be released
December 1 and that the software and hardware would be available
separately. I wrote to them for details but have yet to receive a
reply - so if you're out there AMBIS -when can we have the
program and how much does it cost?

While we're waiting for Ambis to come up with the goods, I've
given the problem to a final year student here who is having a go
at writing a program. If it works, I'll post it - so you better
get that program out soon Ambis!

Johnjoe McFadden
Department of Microbiology
University of Surrey, Guildford, Surrey, UK.