Path: utzoo!utgpu!watserv1!watmath!uunet!bionet!haploid!jeh
From: jeh@haploid (Jamie Hayden)
Newsgroups: bionet.molbio.genbank.updates
Subject: (none)
Message-ID: <9003162321.AA13028@haploid.LANL.GOV>
Date: 16 Mar 90 23:21:28 GMT
Sender: daemon@genbank.BIO.NET
Lines: 59

LOCUS       RATOTCB       409 bp ss-mRNA            ROD       02-MAY-1986
DEFINITION  Rat (Sprague-Dawley) ornithine carbamoyltransferase mRNA.
ACCESSION   K03041
KEYWORDS    ornithine carbamoylase; ornithine carbamoyltransferase.
SOURCE      Rat (Sprague-Dawley) liver, cDNA to mRNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; 
            Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   McIntyre,P., Graf,L., Mercer,J., Peterson,G., Hudson,P. and
            Hoogenraad,N.
  TITLE     A highly basic N-terminal extension of the mitochondrial matrix
            enzyme ornithine transcarbamylase from rat liver
  JOURNAL   FEBS Lett. 177, 41-46 (1984)
  STANDARD  full staff_review
FEATURES       from  to/span     description
    pept        104  >   409     preornithine carbamoyltransferase
    sigp        104      199     ornithine carbamoyltransferase signal peptide
    matp        200  >   409     ornithine carbamoyltransferase
    mRNA    <     1  >   409     OTC mRNA
BASE COUNT      121 a    100 c     91 g     97 t
ORIGIN      120 bp upstream of BamHI site; X-chromosome.
        1 tgcaactgaa atgcattctc tagcttgcca gtggccccca ctgcctgcct gcctgcagga
       61 actactctag acatagattc ctcctccact ctagcaagag aagatgctgt ctaatttgag
      121 gatcctgctc aacaaggcag ctcttagaaa ggctcacact tccatggttc gaaattttcg
      181 gtatgggaag ccagtccaga gtcaagtaca gctgaaaggc cgtgacctcc tcaacctgaa
      241 gaacttcaca ggagaggaga ttcagtacat gctatggctc tctgcagatc tgaaattcag
      301 gatcaaacag aaaggagaat acttgccttt attgcaaggg aaatccttag ggatgatttt
      361 tgagaaaaga agtactcgaa caagactgtc cacagaaaca cgcttcgct
//
LOCUS       YSCHBVREC      65 bp ds-DNA             SYN       02-JUL-1984
DEFINITION  synthetic recombined yeast (s.cerevisiae) promoter and hepatitis b
            hbsag gene.
ACCESSION   J02560
KEYWORDS    alcohol dehydrogenase; dehydrogenase.
SOURCE      synthetic plasmid phbs-16 expressed in yeast (s.cerevisiae)
            xv610-8c.
  ORGANISM  Artificial gene
            Artificial sequences; Genes.
REFERENCE   1  (bases 1 to 65)
  AUTHORS   Valenzuela,P., Medina,A., Rutter,W.J., Ammerer,G. and Hall,B.D.
  TITLE     synthesis and assembly of hepatitis b virus surface antigen
            particles in yeast
  JOURNAL   Nature 298, 347-350 (1982)
  STANDARD  full staff_review
COMMENT     expression of hepatitis b gene in yeast system offers possible
            value as vaccine. plasmid phbs-16 consists of the following
            components (5'-3' on hbsag + strand): pbr322--yeast alcohol
            dehydrogenase (adhi) promoter--ecori linker--hbv surface antigen
            gene (hbsag) and flanks--ecori linker--yeast 2 micron plasmid
            replication origin--yeast trp1 gene--pbr322.
FEATURES       from  to/span     description
    recomb       19       20     yeast adhi promoter/ecori linker
    recomb       27       28     ecori linker/hbv surface antigen gene
BASE COUNT       21 a     16 c     16 g     12 t
ORIGIN      adh1 promoter region, 54 bp upstream of hsa
        1 agcatacaat caactatctg gaattcccga ggactgggga ccctgtgacg aacatggaga
       61 acatc
//