Path: utzoo!utgpu!watserv1!watmath!uunet!wuarchive!usc!apple!bionet!manvax.bitnet!SCF7 From: SCF7@manvax.bitnet ("MYRNA E. WATANABE") Newsgroups: bionet.general Subject: Tissue Culture Course Message-ID: <9004120411.AA27304@genbank.bio.net> Date: 12 Apr 90 04:12:00 GMT Sender: daemon@genbank.BIO.NET Lines: 54 The Biotechnology Program of Manhattan College and the College of Mt. St. Vincent is sponsoring: A SHORT COURSE IN BASIC TISSUE CULTURE JUNE 4-8, 1990 9:00 AM-5:00 PM This short course in the culturing of mammalian cells is presented by means of hands-on procedures and demonstrations. All the essential apsects and contemporary methods of growing cells in culture will be covered. There will be lectures on the cultured cell, monolayer and suspension culture systems, contamination, contemporary methods of cell identification, and production of monoclonal antibodies. Hands-on procedures will include pipetting, media preparation and filtration, basic phase contrast microscopy, the hemocytometer method of cell counting, cryogenic preservation, culturing anchorage dependent cells, primary cell culturing, cell identification using immunohistofluorescence, karyotyping, and mycoplasma detection. Demonstrations on the use of cryogenic equipment, various types of culture vessels, DNA characterization, two dimensional gel electrophoresis of cultured cell proteins emphasizing labeling, isoelectric focusing, polyacrylamide gel electrophoresis and autoradiography will also be presented. GUEST FACULTY: John Ryan, University of Connecticut Dr. Peter Del Vecchio, Albany Medical College Dr. Wallace L. McKeehan, W. Alton Jones Cell Science Center Dr. Martha Weinstein and Dr. Peter Benn, Diagenetics Laboratories Dr. Michael Gabridge, Bionique Laboratories Dr. James Haley, College of Mt. St. Vincent & Albert Einstein College of Medicine Ellyn Fischberg-Bender, Roche Diagnostic Systems Registration (lunch optional) $850 Non-refundable reservation fee applicable to registration $100 For additional information on the course schedule and on-campus housing, contact the Course Director: Dr. Frances M. Cardillo Director, Biotechnology Manhattan College Mount St. Vincent Campus Bronx, New York 10471 USA Tel.: 212-549-8000 ext. 367 or e-mail: Dr. Myrna Watanabe, scf7@manvax.bitnet REGISTRATION DEADLINE: MAY 1, 1990 MANHATTAN COLLEGE AND THE COLLEGE OF MOUNT ST. VINCENT RESERVE THE RIGHT TO CANCEL COURSES WITH INSUFFICIENT ENROLLMENT.