Path: utzoo!attcan!uunet!snorkelwacker!apple!bionet!daemon From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Database Update Message-ID: <9004111728.AA12948@haploid.lanl.gov.LANL.GOV> Date: 11 Apr 90 17:28:40 GMT Sender: daemon@genbank.BIO.NET Distribution: bionet Lines: 300 Approved: lear@genbank.bio.net Checksum: 32164 17 LOCUS ECOTRWA 76 bp ss-tRNA RNA 15-SEP-1989 DEFINITION E.coli Trp-tRNA. ACCESSION M24301 KEYWORDS transfer RNA-Trp. SOURCE E.coli tRNA. ORGANISM Escherichia coli Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively anaerobic rods; Enterobacteriaceae. REFERENCE 1 (bases 1 to 76) AUTHORS Eisenberg,S.P., Yarus,M. and Soll,L. TITLE The effect of an Escherichia coli regulatory mutation on transfer RNA structure JOURNAL J. Mol. Biol. 135, 111-126 (1979) STANDARD simple staff_entry COMMENT In the TrpX mutation the base at position 37 ("a") is not modified. In the "uga" suppressor mutation base 24 "g" is changed to "a". FEATURES from to/span description tRNA 1 76 Phe-tRNA modified 16 16 d modified 17 17 d modified 20 20 d modified 32 32 cm anticdn 34 36 Phe-tRNA anticodon cca modified 37 37 ms2i6a modified 46 46 gm7 modified 55 55 psi BASE COUNT 14 a 21 c 24 g 17 t ORIGIN 5' end of mature tRNA. 1 aggggcgtag ttcaattggt agagcaccgg tctccaaaac cgggtgttgg gagttcgagt 61 ctctccgccc ctgcca // LOCUS ECOTRWSUP 76 bp ss-tRNA RNA 15-SEP-1989 DEFINITION E.coli Trp-tRNA suppressor mutant. ACCESSION M25066 KEYWORDS transfer RNA-Trp suppressor. SOURCE E.coli tRNA. ORGANISM Escherichia coli Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively anaerobic rods; Enterobacteriaceae. REFERENCE 1 (bases 1 to 76) AUTHORS Eisenberg,S.P., Yarus,M. and Soll,L. TITLE The effect of an Escherichia coli regulatory mutation on transfer RNA structure JOURNAL J. Mol. Biol. 135, 111-126 (1979) STANDARD simple staff_entry COMMENT In the TrpX mutation the base at position 37 ("a") is not modified. In Trp-tRNA base 24 "a" is changed to "g". FEATURES from to/span description tRNA 1 76 Phe-tRNA modified 16 16 d modified 17 17 d modified 20 20 d modified 32 32 cm anticdn 34 36 Phe-tRNA anticodon cca modified 37 37 ms2i6a modified 46 46 gm7 modified 55 55 psi BASE COUNT 15 a 21 c 23 g 17 t ORIGIN 5' end of mature tRNA. 1 aggggcgtag ttcaattggt agaacaccgg tctccaaaac cgggtgttgg gagttcgagt 61 ctctccgccc ctgcca // LOCUS ECOTRWX 76 bp ss-tRNA RNA 15-SEP-1989 DEFINITION E.coli Trp-tRNA TrpX mutation. ACCESSION M25067 KEYWORDS transfer RNA-TrpX. SOURCE E.coli tRNA. ORGANISM Escherichia coli Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively anaerobic rods; Enterobacteriaceae. REFERENCE 1 (bases 1 to 76) AUTHORS Eisenberg,S.P., Yarus,M. and Soll,L. TITLE The effect of an Escherichia coli regulatory mutation on transfer RNA structure JOURNAL J. Mol. Biol. 135, 111-126 (1979) STANDARD simple staff_entry COMMENT In the Trp-tRNA the base at position 37 ("a") is modified, whereas it is not modified in the TrpX mutation. In the suppressor Trp-tRNA base 24 "g" is changed to "a". FEATURES from to/span description tRNA 1 76 Phe-tRNA modified 16 16 D modified 17 17 D modified 20 20 D modified 32 32 Cm anticdn 34 36 Phe-tRNA anticodon cca modified 46 46 Gm7 modified 55 55 psi BASE COUNT 14 a 21 c 24 g 17 t ORIGIN 5' end of mature tRNA. 1 aggggcgtag ttcaattggt agagcaccgg tctccaaaac cgggtgttgg gagttcgagt 61 ctctccgccc ctgcca // LOCUS HCYSA 313 bp ds-DNA SYN 15-MAR-1990 DEFINITION Human cystatin A gene. ACCESSION D00404 KEYWORDS cystatin A; cysteine-proteinase inhibitor. SOURCE Artificial gene. ORGANISM Artificial gene Artificial sequences; Genes. REFERENCE 1 (bases 1 to 313) AUTHORS Kaji,H., Kumagai,I., Takeda,A., Miura,K. and Samejima,T. TITLE Studies on chemical synthesis of human cystatin A gene and its expression in Escherichia coli JOURNAL J. Biochem. 105, 143-147 (1989) STANDARD simple staff_entry COMMENT Submitted in computer readable form by H. Kaji on 14-Apr-1989. The expression plasmid for cystatin A was constructed by introducing the synthetic gene downstream of the tac promoter of an E. coli plasmid which is a derivative of pKK223-3 and the gene was expressed in E. coli JM109 without IPTG-induction. FEATURES from to/span description pept 6 302 cystatin A refnumbr 6 8 numbered codon 1 in [1] BASE COUNT 96 a 74 c 83 g 60 t ORIGIN 1 aattcatgat cccgggaggc ttaagcgaag ccaagccagc tacaccggag atccaggaga 61 tcgtcgacaa ggttaagccg cagctcgagg agaagaccaa cgagacatat ggtaagcttg 121 aggccgttca gtataagacc caggttgttg ccggcaccaa ctactacatc aaagttcgtg 181 ctggcgacaa caaatacatg catctgaagg tgttcaaaag cttacctggc cagaacgaag 241 atctggtact gactgggtac caggtcgaca agaacaagga cgacgagtta actggcttct 301 gatgaattct gca // LOCUS HUMPRG 1182 bp ss-mRNA PRI 15-MAR-1990 DEFINITION Human PRG gene (alt. hpg) encoding hemopoietic proteoglycan core protein, complete cds. ACCESSION X17042 M25538 KEYWORDS hemopoietic PG core protein. SOURCE Human adult hematopoietic myeloid cell, cDNA to mRNA, clone D-D2. ORGANISM Homo sapiens Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae. REFERENCE 1 (bases 1 to 1182) AUTHORS Stellrecht,C.M. and Saunders,G.F. TITLE Nucleotide sequence of a cDNA encoding a hemopoietic proteoglycan core protein JOURNAL Nucleic Acids Res. 17, 7523-7523 (1989) STANDARD full staff_review COMMENT Draft entry and computer-readable copy of sequence [1] kindly provided by C.M.Stellrecht, 27-JUL-1989. [1] Author Address: C.M.Stellrecht, University of Texas, M.D.Anderson Cancer Center, Department of Biochemistry and Molecular Biology, Box 117, 1515 Holcomb Blvd., Houston, TX, 77054. FEATURES from to/span description pept 25 501 proteoglycan core protein precursor /nomgen="PRG" /map="10" /hgml_locus_uid="LU0037W" sigp 25 105 proteoglycan core protein signal peptide matp 106 498 proteoglycan core protein signal 279 315 CS substitution signal rpt 304 357 Ser-Gly repeat BASE COUNT 359 a 223 c 224 g 376 t ORIGIN Chromosome 10q22.1. 1 gaattccgct agactaagtt ggtcatgatg cagaagctac tcaaatgcag tcggcttgtc 61 ctggctcttg ccctcatcct ggttctggaa tcctcagttc aaggttatcc tacgcagaga 121 gccaggtacc aatgggtgcg ctgcaatcca gacagtaatt ctgcaaactg ccttgaagaa 181 aaaggaccaa tgttcgaact acttccaggt gaatccaaca agatcccccg tctgaggact 241 gacctttttc caaagacgag aatccaggac ttgaatcgta tcttcccact ttctgaggac 301 tactctggat caggcttcgg ctccggctcc ggctctggat caggatctgg gagtggcttc 361 ctaacggaaa tggaacagga ttaccaacta gtagacgaaa gtgatgcttt ccatgacaac 421 cttaggtctc ttgacaggaa tctgccctca gacagccagg acttgggtca acatggatta 481 gaagaggatt ttatgttata aaagaggatt ttcccacctt gacaccaggc aatgtagtta 541 gcatatttta tgtaccatgg ttatatgatt aatcttggga caaagaattt tatagaaatt 601 tttaaacatc tgaaaaagaa gcttaagttt tatcatcctt ttttttctca tgaattctta 661 aaggattatg ctttaatgct gttatctatc ttattgttct tgaaaatacc tgcatttttt 721 ggtatcatgt tcaaccaaca tcattatgaa attaattaga ttcccatggc cataaaatgg 781 ctttaaagaa tatatatata tttttaaagt agcttgagaa gcaaattggc aggtaatatt 841 tcatacctaa attaagactc tgacttggat tgtgaattat aatgatatgc cccttttctt 901 ataaaaacaa aaaaaaaata atgaaacaca gtgaatttgt agagtggggg tatttgacat 961 attttacagg gtggagtgta ctatatacta ttacctttga atgtgtttgc agagctagtg 1021 gatgtgtttg tctacaagta tgattgctgt tacataacac cccaaattaa ctcccaaatt 1081 aaaacacagt tgtgctgtca atacctcata ctgctttacc tttttttcct ggatatctgt 1141 gtattttcaa atgttactat atattaaagc agaaatataa cc // LOCUS SUSMTA 4191 bp ds-DNA INV 15-MAR-1990 DEFINITION S.purpuratus metallothionein-A (SpMTA) gene, complete cds. ACCESSION M30606 KEYWORDS metal binding protein; metallothionein; metallothionein-A. SOURCE S.purpuratus sperm DNA, clone lambda-MT206. ORGANISM Strongylocentrotus purpuratus Eukaryota; Animalia; Eumetazoa; Echinodermata; Echinozoa; Echinoidea; Echinacea; Echinoida; Strongylocentrotidae. REFERENCE 1 (bases 1 to 4191) AUTHORS Harlow,P., Watkins,E., Thornton,R.D. and Nemer,M. TITLE Structure of an ectodermally expressed sea urchin metallothionein gene and characterization of its metal-responsive region JOURNAL Mol. Cell. Biol. 9, 5445-5455 (1989) STANDARD full staff_review COMMENT Draft entry and computer-readable sequence for [1] kindly submitted by M.Nemer, 11-DEC-1989. FEATURES from to/span description pept 757 787 metallothionein-A, exon 1 1908 1994 metallothionein-A, exon 2 3080 3156 metallothionein-A, exon 3 pre-msg 679 4154 MTA mRNA and introns IVS 788 1907 MTA, intron A IVS 1995 3079 MTA, intron B IVS 3189 3738 MTA, intron C signal 652 656 TATA box BASE COUNT 1194 a 758 c 821 g 1391 t 27 others ORIGIN 1 gtctacgtaa tttagtatgg aaaaataacc attttatcat acaggttgat catcaatata 61 tcattatgca gtgaacacca tgaatatttc gaaatggagg gatagttgca tttctggcgg 121 agatatccgg gtcgggaatc atctactttt attccccctc cccatttttt tcttcttctc 181 cttgttcttc ttctttttcg tttcttaatc atggtattaa tcatttgcaa atcaattaca 241 tgcctttata tatatataat aatcatggtg ctacatgatt ttgaaatact ttatggaaac 301 cttacgtttt accagacctt gacacaaagt tgacagtgtt acctttatca ttcacttccg 361 tactgtttca gcacatgagc aacactttgt tctttgaggg caaacgtttt gggggagggg 421 agggcatccc acggcgcttt aaaaacaaaa aggatttttg cttcccttcc atatcccgta 481 taatttatcc gacacgagcc tagtcatcac cattcctgat agaagcgtgt gcaaaactag 541 aaaggcccac ctagatcgag ctcctcaggc accgctaaca ggttgcctag ctcgtgccta 601 aacgtggtgt gtttacctat ttgtttgcac acggatattg taatgaaaac atataaaacc 661 ccgaatttca ccagtttttg tcacaagctg aagcgtttga gtgtgattta cattaccttc 721 tgaagaaaca tacaactcga gttcaatttc atcaccatgc ctgatgtcaa gtgtgtctgc 781 tgcaagggta agcaaaaaat cggaatcaat gttctcatta aaataccgag ttacagaata 841 ccgtgtacgt acggtgtagc gtttctagaa acaaaccgct cgatgttacg tgcatacgat 901 gatacgatac agccatggcc atgaccatga gaggcccccg atcacgacac acgcagctag 961 ctagaccgaa ttttccgtct ctttcgttcg ttaagcctct tatttgggta gggtcggctc 1021 tctctttaga gacggaagcc gactagacct agggcgcgtt catgcttgat tttgaaggca 1081 gaatgagcga tttgtaacgc tcatcgtaaa cgctcatcgt aatcgcagtt gcacggtgca 1141 ctgttcatgc tcacttctcc aggcaattct ccttgggtgc cttcagctgg attcgcaaac 1201 ctgtgtgcgc atgctacgag agcccataga ggtcagtgcg ttgaccccat ttaaacgcgt 1261 ttacaaattg taaacgcatc tttttgtgag tttacgatcg gcgatcatga atcggctttt 1321 tcggtgattc tacaaaatca agcatgaacg cgcccctata ctcattccct tgctaacacc 1381 aaggtgcatt tatttttann nntatnccna naannncccc aaatgtcatt ttacctcgtc 1441 gcatgttata ttgtaaatta ggcctaggct ataataaatc ggttccactt accaacttga 1501 ctgaaataat ttaatggtcc ctttttagga aattatgggt ctgaaatcga aatccatgtt 1561 gaaaatcttg caaaacgtat tttaactctc gctgcgcacg acgtaatcta aatctcatcc 1621 cttctagtct acaccgaagg caaaatgatt atgaaggaaa aactgtgcgc gagcttcacg 1681 ttcgttatca cagtttttct tcataatcat tttcatgttt tcttgcccgt ttcttcatac 1741 ttgttacatc aacagttatg tcaataacta agtttttacc aacaagcact gctggatttg 1801 tggtttcaaa ttgcaagatt gttattactt ttttaaaatt ttatatcatt atatagacta 1861 tatatatata tatatatata tatttttttt tttaattctg aacacagagg gcaaggagtg 1921 tgcctgcttt ggacaagatt gctgtaaaac tggtgaatgc tgcaaggatg gaacctgctg 1981 tggaatatgc acaagtaagc ctttcatcat tttcagtgaa ccctttaaaa ttaaagcagg 2041 cgcgtacgag gattttttaa gggggggggg ggggggttta acatttataa atcgggccga 2101 aaattctgca acgactcagc cgaccgctga ataagtgnng gggggggggg gggtagtaag 2161 aggacacttt ttattttttt ttggtctcga aattagaaaa tttgacattt tgctccgttg 2221 gggggggggg ggggaaggtc agctttttta ggtcagccat gggaattttt ttttttaata 2281 aaaaaaaaaa acatccagan nnnggggggg ggtttttaag gaaatttagg gggggggttt 2341 acacacaaaa aattaccaaa gggtgggggt ttaaccnnnn nnnncccctg tgtaagcncc 2401 tgcatcccag taattaaggc ttttttatac taaactgttt acacaaggct tttaaaagta 2461 tttatattca tagaaaaaaa ttgtcccaat tgctcatgca tttttttaat ttctttttac 2521 tctcaccttt aaatgggtgc aggcttaaat gtcaggctaa ctggcaatga ttatttgtgc 2581 tcattatcaa tattctagtg tatccatggt tttgttatgg tcgggggggg gggatctgac 2641 aaaaaatatt gacagccaag aatataggtc tatatgattt gtcagggaga tatgaatttc 2701 acatctccta ggttttattg ttgttgtcac caattaaatt attttaaaaa tcactgacaa 2761 gcttggaaaa ggattttgaa aataatttaa aacatgttca tctcattttc ctggtgaatt 2821 ttgggtctga aaagcgctat ataagaacta gctattatta tatatatata tatatatata 2881 taattagatt ttttattttt ttggaggggg gggggggggn ctttttccat gtatgcctaa 2941 atgaggggcg cagaccaatt gtgcccttta tatatcctgc tgtgtagtct gcatgaaaga 3001 gaaaatgcag ttataccaac aatagaatag agacatttca atttgatgtt attacttggt 3061 taattttatt tgtccacaga cgctgcgtgc aagtgtgcta atggctgcaa gtgtggaagc 3121 ggctgctcat gcactgaggg gaactgtgca tgctagacga gcttggccct ttgctatagg 3181 gatggcaggt gagattttat ttattttcat ggctacatag taaaaattgt ttccaggcaa 3241 aaacgttagc tagttatggg atatgacagg gtctgaaatg taagactgta gatttgcact 3301 gcataaagct gtgccatcat ttttttttaa atagaatttt ccgagagagc atcaaaatta 3361 atgtccaaat gtcgaaagcc aactgaatat gttaaatatt ggctgagatg tgaccatctg 3421 aataaatagg ttccctatag gaactcagtg ttattttgct gtggttcata acatcacgna 3481 ccagagcaaa ataacactga gttcctacac aaaatctatt tattacatta ctgcattgcg 3541 atatacaaaa aaggtaaaat tagaaaatct ttgcaagtta tggcaatatg tatagacctt 3601 ccgtgtcttt catgactttt gtatattttt ttcaacaacc ggaacaaatt cattaaccat 3661 ttctgtgttt tcatactttt ttgtaaaaat attttcaagc attttgtttt tctctccgtg 3721 tctgtttctc ttctacagat tgagcataca gctgatgaag tcactcagcg catctgccaa 3781 catgctccaa gcagacctga ctttgtatat actttcgacc cgatgtatta tattttttgt 3841 tgtaagatag ttgtatatta agttaattta atctttattt ttttctgaaa atatgagact 3901 aattggcatt aataggacta cttaaacaac aattatttat taaggctgac agtaatgcct 3961 gatagaattt cacttcattt tttgctgccc tgttttgtaa attcttcagt ctgtttctaa 4021 cttctatcaa gttgcaaatt tggaatgaaa ataataataa taatacatgt ggagagtact 4081 tgtagtgcgc aaactccaca tgactaatgt gttaaagact cgctcatgat taaatttaaa 4141 taatgctaca aagtatattc agtatgcgtt gcttagtttt ttctcatgat t // LOCUS SYNCYSTA 318 bp ds-DNA SYN 15-MAR-1990 DEFINITION Artificial cystatin alpha gene. ACCESSION X13226 KEYWORDS artificial gene; cystatin; cysteine-proteinase inhibitor. SOURCE Artificial gene. ORGANISM Artificial gene Artificial sequences; Genes. REFERENCE 1 (bases 1 to 318) AUTHORS Katunuma,N., Yamato,M., Kominami,E. and Ike,Y. TITLE Total synthesis of the cystatin alpha gene and its expression in E. coli JOURNAL FEBS Lett. 238, 116-118 (1988) STANDARD simple automatic COMMENT *source: library=pBR322; clone=pTPI-010; Data kindly reviewed (23/5/89) by Katunuma N. FEATURES from to/span description pept 6 317 cystatin alpha (AA 1 - 103) BASE COUNT 99 a 63 c 76 g 80 t ORIGIN 1 aattcatgga cccgggtact actggtatcg ttggtggtgt ttctgaagct aaaccggcta 61 ctccggaaat ccaggaagtt gctgacaaag ttaaacgtca gctggaagaa aaaactaacg 121 aaaaatacga aaaattcaaa gttgttgaat acaaatctca ggttgttgct ggtcagatcc 181 tgttcatgaa agttgacgtt ggtaacggtc gtttcctgca catgaaagtt ctgcgtggtc 241 tgtctggtga cgacgacctg aaactgctgg actaccagac taacaaaact aaaaacgacg 301 aactgactga cttctagg //