Path: utzoo!attcan!uunet!munnari.oz.au!murdu!morrow@murdu.ucs.unimelb.edu.au From: morrow@murdu.ucs.unimelb.edu.au (Christopher Morrow) Newsgroups: bionet.molbio.methds-reagnts Subject: REA patterns Message-ID: <1866@murdu.oz> Date: 29 May 90 11:23:11 GMT Sender: morrow@murdu.oz Organization: Vet School, University of Melbourne Lines: 20 I have been very interested in photography of REA patterns of bacteria and further processing by computers. Recently I have looked at improving photograph resolution and manipulating constrast (NAR 17:6751) and I have tried the NSCA gelreader program. The gel reader program is fantastic taking a digitized picture and identifying lanes and bands and generating rf values. It is designed for DNA but could easily be adapted for protein work. It handles standards. It runs on a Mac with a colour card (ours is a Mac II) and it is recommended to use a HP Scan jet plus for digitizing as the apple scanner doesnot have the required grey scale. What I was thinking was could a scanner be modified to scan gels? Either by putting a UV source above the gel on the scan bed or altering the laser/dye/glass or just using it as is. I wanted to try to stop buying film and was thinking of a video camera but this idea appeals to me. Perhaps HP could design a water proof scanner. Chris Morrow