Path: utzoo!utgpu!watserv1!watmath!uunet!cs.utexas.edu!usc!apple!bionet!sysh.surrey.ac.uk!MBY134
From: MBY134@sysh.surrey.ac.uk
Newsgroups: bionet.molbio.methds-reagnts
Subject: (none)
Message-ID: <9005291727.AA13840@genbank.bio.net>
Date: 29 May 90 17:12:48 GMT
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                     PCR REPRODUCIBILITY

We are presently having difficulty with day to day
reproducibility of the PCR. One day it works fine, the next,
with the same reagents, it seems to either not work at all or
the sensitivity is down 100-fold. We are using the HYBAID
THERMAL REACTOR, Taq polymerase from GIBCO-BRL, frozen stocks
of primers made on a Applied Biosystems synthesizer, buffer
(with W1 detergent) and a standard dilute DNA prep.
Has anyone had similar problems?