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From: rabani@sdbio2.ucsd.edu (Ely Rabani/D Smith)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: king-size-cloning
Message-ID: <11692@sdcc6.ucsd.edu>
Date: 2 Jul 90 11:02:08 GMT
References: <9006261058.AA25702@genbank.bio.net>
Sender: news@sdcc6.ucsd.edu
Reply-To: rabani@sdbio2.ucsd.edu (Ely Rabani/D Smith)
Organization: University of California, San Diego
Lines: 13

In article <9006261058.AA25702@genbank.bio.net> FRED@blekul13.bitnet (fred) writes:
>The fun thing is that she has managed to clone a 9 kB SacI fragment
>into the 2.7 kB pUC vector and that the thing is behaving completely
>normal in our E.Coli as if it was not 11.7 kB big. Our question now:
>is this the world record or are we too optimistic? Unless someone
>says that these vectors can do more than expected we want to believe
>that this is an exceptionally big insert for pUC. Is this

Don't really know about pUC, but supposedly inserts >40kb (!!!!!) have
been successfully cloned into M13...saw this cited someplace in Methods
in Enzymology, think the reference was Michael Bevan.  

ER