Path: utzoo!utgpu!watserv1!watmath!uunet!snorkelwacker!bionet!sysh.surrey.ac.uk!MBY134
From: MBY134@sysh.surrey.ac.uk
Newsgroups: bionet.molbio.methds-reagnts
Subject: (none)
Message-ID: <9008101150.AA29269@genbank.bio.net>
Date: 10 Aug 90 11:01:40 GMT
Sender: daemon@genbank.BIO.NET
Lines: 13

Does anyone have a good method for extracting good yields of DNA from
a 50Kb recombinant cosmid clone - in pHC79 - a ColE1 based lambda cosmid.
Alkaline extraction of plasmid DNA gives very low yields - too low to
band on caesium chloride. Any ideas?

Johnjoe McFadden
Department of Microbiology
University of Surrey
Guildford, Surrey, UK.

       'Ever tried? Ever failed? No matter. Try again. Fail again.
        Fail better!
         ACCCCCCCCCCC'BDDDDD           Samual Becket