Path: utzoo!attcan!uunet!snorkelwacker!bionet!BIOVAX.RUTGERS.EDU!FRIEDMAN
From: FRIEDMAN@BIOVAX.RUTGERS.EDU
Newsgroups: bionet.molbio.methds-reagnts
Subject: How do you clone large fragments in large vectors?
Message-ID: <4D1C8335FF3F6011D3@mbcl.rutgers.edu>
Date: 14 Aug 90 15:46:00 GMT
Sender: daemon@genbank.BIO.NET
Lines: 10

I have been trying for a while to clone several random fragments that I obtained
from D. melanogaster into a 16kb vector.  The fragments (6-12+kb) are already 
cloned into pUC19 vectors at the site (KpnI) which I want to use to clone into 
the 16 kb vector.  I have phosphotased the vector and it seems to not self 
ligate. The colonies I do get do not appear to be the product of the ligation. 
Any suggestions as to concentrations buffers other than standard (Maniatis) 
would be helpful
Rich
Friedman@MBCL.rutgers.edu
Friedman@biovax.rutgers.edu