Path: utzoo!utgpu!watserv1!watmath!uunet!bionet!VAX5.CIT.CORNELL.EDU!UAPJ From: UAPJ@VAX5.CIT.CORNELL.EDU Newsgroups: bionet.molbio.methds-reagnts Subject: I need a plasmid to express SP6 RNA pol in E. coli Message-ID: <4C018BF39E5F204699@vax5.cit.cornell.edu> Date: 16 Aug 90 02:31:00 GMT Sender: daemon@genbank.BIO.NET Lines: 16 I would like to express the SP6 RNA polymerase in an E. coli strain that contains a pBR322 based plasmid. Does anyone out there have an expression plasmid ( preferably inducible) that I could use? Any other ideas on how to express the SP6 polymerase would be most welcome. =================================================== " Steve Passmore " Biochemistry,etc " Cornell University UAPJ@VAX5.CIT.CORNELL.EDU " Ithaca, NY " " ===================================================