Path: utzoo!utgpu!news-server.csri.toronto.edu!mailrus!wuarchive!zaphod.mps.ohio-state.edu!swrinde!emory!mephisto!mcnc!beguine!ocean!joan
From: joan@ocean.med.unc.edu (Joan Shields)
Newsgroups: sci.bio
Subject: Re: Membrane Proteins
Keywords: c-fos, GTP-binding proteins
Message-ID: <915@beguine.UUCP>
Date: 24 Aug 90 14:36:54 GMT
References: <840@beguine.UUCP>
Sender: usenet@beguine.UUCP
Reply-To: joan@uncmed.med.unc.edu (Joan Shields)
Organization: UNC-CH School of Medicine
Lines: 21

In article <840@beguine.UUCP> joan@uncmed.med.unc.edu () writes:
>If there is anyone out there in netland who has worked with membrane
>proteins (esp. in assay situations), PLEASE, I NEED HELP AND LOTS OF
>ADVICE!!

've gotten lots of replies to my query and I would like to thank all who
have answered my plea for help.

I guess I should have been more detailed about what I am interested in.  I
am working with c-fos proteins in rat brains.  I have been using an ELISA
assay, which I have had little trouble with, however I have been having
problems finding a detergent that will either not interfere with the ELISA
or that is easily removed.  References, and of course any hints and
advice, will be happily received.

Thanks again.

Joan Shields
UNC-CH
"There's rumors in the loading bay there's anger in the town
Somebody blew the whistle and the walls came down"