Path: utzoo!utgpu!watserv1!ria!uwovax!37_410
From: 37_410@uwovax.uwo.ca
Newsgroups: bionet.molbio.proteins
Subject: anomalous protein migration on PAGE ?
Message-ID: <6877.26e56295@uwovax.uwo.ca>
Date: 6 Sep 90 00:39:49 GMT
Lines: 11



Does anyone have any experience with proteins that migrate significantly slower
by SDS-PAGE than their DNA sequence predicts?  I have a 22.3 Kd ORF that yields
a 35 Kd polypeptide on gels.  I would like to know if someone has similar
experience or some possible explanation(s).

Jim Gordon
University of Western Ontario,
Ontario, Canada
email: (care of) T.LINN@uwovax.uwo.ca