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From: v151luy2@ubvmsa.cc.buffalo.edu (Scott D McGinnis)
Newsgroups: sci.bio
Subject: Re: Membrane Proteins
Keywords: c-fos, GTP-binding proteins
Message-ID: <35367@eerie.acsu.Buffalo.EDU>
Date: 9 Sep 90 23:27:43 GMT
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In article <915@beguine.UUCP>, joan@ocean.med.unc.edu (Joan Shields) writes...
>In article <840@beguine.UUCP> joan@uncmed.med.unc.edu () writes:
>>If there is anyone out there in netland who has worked with membrane
>>proteins (esp. in assay situations), PLEASE, I NEED HELP AND LOTS OF
>>ADVICE!!
> 
	Actually, I tried to E-Mail you but for some reason 
you are out of my jurisdiction.
	I have alot of experience w/ ELISAs and have worked
purifying the Rhodopsin/GTP-ase/PDE-ase sytem *functionally*
What I don't understand is the nature of you ELISA 
problems? Be exact I can understand any tech. jargon. 
As for the C-fos system which I am unfamiliar, can you 
tell me weither it is the G-protein in the cascade or
does it play another role? Is the detergent necessary 
to purify it, or is the detergent in the ELISA wash 
buffer (your wording is unclear)? Do you need help 
purifying other enzymes such as the membrane protein
in your system which activates the G-protein and the
Adenylate Cyclase (if that's what it is)? And I am 
interested in *how* you intend to assay this system:
for instance, the formation of cAMP ect.? Finally,
does the system need to be reconstituted into mem-
brane vesicles in order to be functional (a potent-
ial pain in the rump right here)?
	I know that this is a lengthy cross-exam. but I
can handle any technical jargon I am quite familiar
with both of these systems as I've said. You may 
want to E-mail me direct so as not to clutter up
 the newsgroup.
			Scott
			V151LUY2@ubvmsa