Path: utzoo!utgpu!watserv1!watmath!uunet!snorkelwacker!bionet!wfeb2.bitnet!MELAN
From: MELAN@wfeb2.bitnet
Newsgroups: bionet.molbio.methds-reagnts
Subject: (none)
Message-ID: <9010012302.AA04669@genbank.bio.net>
Date: 2 Oct 90 00:06:00 GMT
Sender: daemon@genbank.bio.net
Lines: 59


        My message posted to this board the other day requires some
clarification and also raises additional questions concerning addresses and
the origin of messages.  The posting that I was attemtping to respond to was
that from M.N. concerning plant chromosome condensation.  I responded to the
address listed at the top of the header which was edu% KBIO11%DKLUNIO1@pucc.
princeton.edu.  The message kept bouncing back to me from Princeton with the
error message that DKLUNIO1 was an invalid username.  So wondering *Who is
M.N. at Princeton?*, I posted the message concerning *proper* signatures.  Upon
closer inspection of the header I saw that the message was SENT to pucc at
princeton from IRLEARN (this is in line 15 of the header!) without the
slightest clue of the ultimate origin of the posting.  I'm now left wondering
*Who is M.N. in EUROPE?*.  So my question now is:  Is there any way to trace
back messages coming over from IRLEARN if there is no *return address*?


    And for M.N. whoever you are, wherever you are here is my response to
your request.




From:   WFEB2::MELAN        MELISSA MELAN (508) 842-8921 28-SEP-1990 17:29
To:     Orig_To! KBIO11%DKLUNIO@pucc.princeton.edu, MELAN
Subj:   Plant Chromosome Condensation


Dear M.N. (next time please give your name!),

        In response to your request concerning plant chromosome condensation,
I have a few suggestions and a few questions.  First of all, what plant are
you working with and what is your interest in chromosome condensation?  If you
are able to get a good isolate of the plant's nuclei then you may be able to
make use of the Xenopus egg extract system referenced below.  This may be a
very elegant way of getting at your questions about chromosome condensation.
I also have it on good authority (Jim Maller, personal communication) that it
may also work with purified DNA.  This, however, would give *chromosomes*
which contain plant DNA and frog histones, etc. and may not be what you want.
In any case, you should check out the following references:

        Lohka, M.J. and J.L. Maller. 1985. Induction of nuclear envelope
           breakdown, chromosome condensation and spindle formation in cell
           free extracts. J. Cell Biol. 101: 518-523.

        Lohka, M.J. and J.L. Maller. 1988. Induction of metaphase chromosome
           condensation in human sperm by Xenopus extracts. Exp. Cell Res.
           179: 303-309.

        Feiler, H.S. and T.W. Jacobs. 1990. Cell division in higher plants:
          A cdc2 gene, its 34-kDa product and histone H1 kinase activity in
          pea. PNAS 87: 5397-5401.

Good luck with your experiments.  I would be interested in knowing your results.

=============================================================

Melissa Melan     (MELAN@WFEB2):BITNET
Worcester Foundation for Experimental Biology
Shrewsbury, MA