Path: utzoo!attcan!uunet!shelby!snorkelwacker!usc!wuarchive!julius.cs.uiuc.edu!zaphod.mps.ohio-state.edu!pacific.mps.ohio-state.edu!ohstpy!miavx1!miamiu!jahayes From: JAHAYES@MIAMIU.BITNET (Josh Hayes) Newsgroups: bionet.population-bio Subject: Estimation of Gene Flow (was re: true gene flow) Message-ID: <90288.094237JAHAYES@MIAMIU.BITNET> Date: 15 Oct 90 14:42:37 GMT References: <9010131802.AA25850@genbank.bio.net> Organization: Miami University - Academic Computer Service Lines: 39 In a related question, I am planning to embark on a study that the net might be able to discourage or encourage. Here's a brief description: The marine gastropod Coralliophila abbreviata feeds on stony corals in the Western Atlantic. It's a sequential hermaphrodite (protandrous), and produces planktonic larvae after brooding them briefly in the mantle cavity. It's a simple matter to develop a model to predict the adaptive size/age at which an individual should switch from male to female (I can supply references if anyone's interested -- heck, even a manuscript rejected from American Naturalist!), within the constraints of local size/age structure and known fecundity/size relationships. My snails don't do it right; at four out of five sites they're way off the predicted sex ratio. I hope to get some rough idea of gene flow in the critters by using allozyme analysis a la Nei's stats (pace Hillis, 1984). The question is, is this a reasonable way to look for gene flow? Obviously, there are possible results that would vitiate the study, but is this at least a reasonable approach? Planktonic larvae are a real bear to work with, for the simple reason that they're planktonic. Once produced, they pretty much vanish. Then when they recruit to some substrate, there's no way of knowing whether they were PRODUCED there (and thus ought to exihibit locally adaptive phenotypes) or elsewhere (and so might not be adaptive for these local conditions). We're having trouble getting resolution on the gels so far, but I would appreciate any comments or questions about the study. Thanks! Josh Hayes, Zoology Department, Miami University, Oxford OH 45056 voice: 513-529-1679 fax: 513-529-6900 jahayes@miamiu.bitnet, or jahayes@miamiu.acs.muohio.edu I'm back, I'm back! I've been to ancient Greece -- I have proof, look at this grape!