Path: utzoo!attcan!uunet!wuarchive!zaphod.mps.ohio-state.edu!usc!apple!bionet!sysh.surrey.ac.uk!MBY134
From: MBY134@sysh.surrey.ac.uk
Newsgroups: bionet.molbio.methds-reagnts
Subject: (none)
Message-ID: <9010231401.AA01888@genbank.bio.net>
Date: 23 Oct 90 12:53:55 GMT
Sender: daemon@genbank.bio.net
Lines: 14

Does anybody have any particular tips for doing inverse-PCR?
We've tried alterring magnesium conc., dilute ligations to encourage
intramolecular ligation, long extension times - but no product (dispite
direct PCR working on template)
Any ideas welcome.

           "Ever tried? Ever failed?. No matter
            Try again. Fail again. Fail better!
                             Samual Beckett

Johnjoe McFadden
University of Suttey, UkK
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