Path: utzoo!attcan!uunet!crdgw1!rpi!julius.cs.uiuc.edu!wuarchive!cs.utexas.edu!yale!venus!yalevm!maine!laflamme
From: LAFLAMME@MAINE.BITNET
Newsgroups: bionet.molbio.methds-reagnts
Subject: Oligonucleotide probing
Message-ID: <90298.171656LAFLAMME@MAINE.BITNET>
Date: 25 Oct 90 21:16:56 GMT
Organization: University of Maine System
Lines: 8

Does anyone out there in netland have any experience in the use of
oligonucleotide probes to screen total DNA in northern or slot-blots?
I have been using 20 mers to pick out individual members of a family
of plant genes.  It seems that the method is not sensitive enough to
detect these sequences in a sample containing a HUGE excess of
non-hybridizing species.  If anyone could pass along a helpful hint
I would be very happy.  I would prefer not to isolate poly A RNA due
to the extra hassle and the possibility of skewing the final results.