Path: utzoo!attcan!uunet!bionet!rutgers!mcnc!uvaarpa!murdoch!krebs.acc.Virginia.EDU!mkw6d
From: mkw6d@krebs.acc.Virginia.EDU (Martyn K. White)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Oligonucleotide probing
Message-ID: <1990Oct29.213000.24915@murdoch.acc.Virginia.EDU>
Date: 29 Oct 90 21:30:00 GMT
References: <90298.171656LAFLAMME@MAINE.BITNET>
Sender: news@murdoch.acc.Virginia.EDU
Organization: University of Virginia
Lines: 13
Ireallyam: mkw6d

In article <90298.171656LAFLAMME@MAINE.BITNET> LAFLAMME@MAINE.BITNET writes:
>Does anyone out there in netland have any experience in the use of
>oligonucleotide probes to screen total DNA in northern or slot-blots?
>I have been using 20 mers to pick out individual members of a family
>of plant genes.  It seems that the method is not sensitive enough to
(stuff deleted)

Have you thought of PCR.

	Martyn K. White, Box 441, U.Va, Cville VA 22908
	     		(804) 924 8710	
                 mkw6d@krebs.acc.Virginia.EDU
		      mkw6d@Virginia.EDU