Path: utzoo!utgpu!watserv1!watmath!uunet!bionet!PUCC.PRINCETON.EDU!BAIROCH%CH.UNIGE.CMU
From: BAIROCH%CH.UNIGE.CMU@PUCC.PRINCETON.EDU (Amos Bairoch)
Newsgroups: bionet.general
Subject: Re: chaperone proteins
Message-ID: <0A6E2A8EB9DF00103D@cgecmu51.bitnet>
Date: 7 Nov 90 07:20:00 GMT
Sender: daemon@genbank.bio.net
Lines: 49

From: MBBPH@DLVH.DARESBURY.AC.UK
Subject: chaperone proteins

<can anyone direct me to motif(s) found in 'chaperone proteins.
<brendan hughes. univ. of oxford uk.


From current version of PROSITE:

$PS00296; CHAPERONIN
$BEGIN
*************************
* Chaperonins signature *
*************************

Chaperonins[1,2] are proteins implicated in the assembly of oligomeric protein
complexes.  Their role seem to be to assist other polypeptides to  maintain or
assume  conformations  which  permit their  correct  assembly  into oligomeric
structures.  They are  found in  abundance in  chloroplasts, mitochondria  and
prokaryotes.  They show weak ATPase  activity,  and form oligomeric complexes.
The proteins known to belong to the chaperonin family are listed below.

 - The chloroplast RuBisCO subunit  binding-protein  alpha chain, which  binds
   ribulose bisphosphate carboxylase small and  large  subunits  and  which is
   implicated in the assembly of the enzyme oligomer.
 - The mammalian mitochondrial protein P1 (mitonin) whose function is  not yet
   known.
 - The yeast HSP60 protein, a mitochondrial assembly factor.
 - The Escherichia coli groEL protein,  which  is essential for the  growth of
   the bacteria and the assembly of several bacteriophages.
 - The Mycobacterium tuberculosis and leprae 65 Kd antigen, and the heat shock
   protein htpA from Coxiella burnetti, which are probable analogues of groEL.
 - The Chlamydial 57 Kd hypersensitivity antigen (hypB) [3].
 - A hypothetical protein  from Synechococcus 6301 adjacent to an ATP synthase
   subunits locus.

We chose, as a signature pattern  for this group of  proteins, a  rather  well
conserved region of twelve residues.

-Consensus pattern: A-A-x-E-E-x(4)-G(3)
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in SWISS-PROT: NONE.
-Last update: April 1990 / Text revised.

[ 1] Hemmingsen S.M., Woolford C., van der Vies S.M., Tilly K., Dennis D.T.,
     Georgopoulos C.P., Hendrix R.W., Ellis R.J.
     Nature 333:330-334(1988).
[ 2] Gupta R.S., Picketts D.J., Ahmad S.
     Biochem. Biophys. Res. Commun. 163:780-787(1989).


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