Path: utzoo!attcan!uunet!fernwood!apple!petunia!usc!zaphod.mps.ohio-state.edu!uwm.edu!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: E.coli Leu-tRNA-1. Message-ID: Date: 6 Nov 90 13:00:07 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 48 Approved: lear@genbank.bio.net LOCUS ECOTRL1 87 bp ss-tRNA RNA 06-NOV-1990 DEFINITION E.coli Leu-tRNA-1. ACCESSION K01550 KEYWORDS transfer RNA; transfer RNA-Leu. SOURCE Escherichia coli strains B and K12 RNA. ORGANISM Escherichia coli Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively anaerobic rods; Enterobacteriaceae. REFERENCE 1 (bases 1 to 87) AUTHORS Dube,S.K., Marcker,K.A. and Yudelevich,A. TITLE The nucleotide sequence of a leucine transfer RNA from E. coli JOURNAL FEBS Lett. 9, 168-170 (1970) STANDARD full staff_review REFERENCE 2 (bases 1 to 87) AUTHORS Blank,H.-U. and Soell,D. TITLE The nucleotide sequence of two leucine tRNA species from Escherichia coli K12 JOURNAL Biochem. Biophys. Res. Commun. 43, 1192-1197 (1971) STANDARD full staff_review REFERENCE 3 (sites) AUTHORS Yudelevich,A. TITLE modification of Leucine tRNA of Escherichia coli after bacteriophage T4 infection JOURNAL Basic Life Sci. 1, 229-239 (1973) STANDARD full staff_review COMMENT Contributed on tape April 1983 by M.Sprinzl & D.H.Gauss; from their entry 1010 in Nucleic Acids Res. 11, r1-r54 (1983). [1] suggests that the unidentified guanosine derivative at 38 is either 1'-O-methylguanosine or 2'-O-methylguanosine. [2] also sequenced E.coli Leu-tRNA-2. FEATURES from to/span description tRNA 1 87 Leu-tRNA-1 (NAR: 1010) /anticodon="(pos:35..37,aa:Leu)" modified 16 16 d = dihydrouridine modified 17 17 d = dihydrouridine modified 18 18 gm = 2'-O-methylguanosine modified 20 20 d = dihydrouridine modified 38 38 unidentified guanosine derivative (see comment) modified 39 39 f = pseudouridine modified 41 41 f = pseudouridine modified 65 65 t = 5-methyluridine modified 66 66 f = pseudouridine BASE COUNT 15 a 24 c 29 g 19 t ORIGIN 5' end of mature tRNA. 1 gcgaaggtgg cggaattggt agacgcgcta gcttcaggtg ttagtgtcct tacggacgtg 61 ggggttcaag tcccccccct cgcacca // Brought to you by Super Global Mega Corp .com