Path: utzoo!utgpu!watserv1!watmath!uunet!bionet!vme.ccc.nottingham.ac.uk!Chris_Jones
From: Chris_Jones@vme.ccc.nottingham.ac.uk
Newsgroups: bionet.molbio.methds-reagnts
Subject: PCR cloning
Message-ID: <_2_Nov_90_15:54:07_A10C2F@UK.AC.NOTT.VME>
Date: 2 Nov 90 15:54:07 GMT
Sender: lear@genbank.bio.net
Lines: 11

I've just seen an ad for Invitrogens PCR cloning kit where they
claim that PCR products always have a 3' A added on. Does anyone
know anything about this (reference?). I have always done a klenow step
before cloning but didn't realise that the ends were ragged because extra
bases were added on? Quite a few of my applications would be wrecked by an
extra A so any tips about eliminating them (if they exist) would be great.

                                                  Chris Jones
                                                  Biochemistry Dept
                                                  Nottingham Univ.
                                                  U.K.


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