Path: utzoo!attcan!uunet!jarthur!usc!apple!bionet!CSC.FI!WALLEN From: WALLEN@CSC.FI Newsgroups: bionet.molbio.methds-reagnts Subject: re:PCR CLONING Message-ID: <0BB03F5975BFC0B420@CSC.FI> Date: 5 Nov 90 17:54:00 GMT Sender: daemon@genbank.bio.net Lines: 13 Dear Chris, A reference that may be of interest to you is Perkin Elmer's newsletter (Amplifications, May 1990, Issue 4). There is an article called "DNA generated by PCR (...) has non-template nucleotide additions: Implications for cloning PCR products. They suggest using Klenow to remove the extra nucleotide (which probably is an A). Hope this is of some help to you. Mika Wallen Univ. Tampere Finland wallen@csc.fi (Internet) wallen@finfun (EARN/Bitnet) Brought to you by Super Global Mega Corp .com