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From: danielg@uncmed.med.unc.edu
Newsgroups: bionet.molbio.methds-reagnts
Subject: Need human monocyte isolation procedure
Message-ID: <1573@beguine.UUCP>
Date: 9 Nov 90 14:30:12 GMT
Sender: usenet@beguine.UUCP
Reply-To: danielg@uncmed.med.unc.edu ()
Organization: UNC-CH School of Medicine
Lines: 17

We need to isolate *viable* human monocytes from whole blood, with >90%
purity, and highest possible yield, of course :-).  In the past we have
used mono-poly resolving media (Flow Labs), which gives two bands, one
containing polynuclear cells, the other containing mononukes.  We need to
remove the lymphocytes (we have also had a slight problem with some pmn
contamination in the mono layer - poly layer, however, has been quite
pure).  

All suggestions warmly welcomed.  Email or post.

Thanks in advance,

dan
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     S A V E  T H E  W H A L E S  ,  K I L L  O U R  U N B O R N  ??  
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Daniel G. Sinclair - UNC Chapel Hill - Satisfied in Jesus. :-).  Ahhhhhh.


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