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From: kelley@aclcb.purdue.edu (Steve Kelley)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Need human monocyte isolation procedure
Message-ID: <0093F73C.C1E6E720@aclcb.purdue.edu>
Date: 9 Nov 90 16:16:29 GMT
References: <1573@beguine.UUCP>
Sender: news@mentor.cc.purdue.edu
Reply-To: kelley@aclcb.purdue.edu (Steve Kelley)
Organization: Purdue AIDS center
Lines: 22

In article <1573@beguine.UUCP>, danielg@uncmed.med.unc.edu writes:
>
>We need to isolate *viable* human monocytes from whole blood, with >90%
>purity, and highest possible yield, of course :-).  In the past we have


Have you tried flow cytometry?  Purity > 90% should be no problem at all,
but what exactly do you mean by highest possible yield?  Flow will limit
you to looking at a couple thousand cells per second, and picking out 
whatever fraction of the input are monocytes.  

If flow cytometry alone won't get you the numbers you need, perhaps a
two-step procedure will do it?

I believe Larry Arnold is at UNC, in the dept of Micro and Immuno.  He
can fill you in on all the gory details of sorting.

Steve Kelley

    Name: Steve Kelley
Internet: kelley@aclcb.purdue.edu
   Phone: 317 494-8638


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