Path: utzoo!utgpu!watserv1!watmath!uunet!nih-csl!lhc!bionet!agate!ucbvax!ucsd!usc!zaphod.mps.ohio-state.edu!think.com!yale!venus!yalevm!maine!laflamme
From: LAFLAMME@MAINE.BITNET
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Problem with lambda GT10
Message-ID: <90318.171508LAFLAMME@MAINE.BITNET>
Date: 14 Nov 90 22:15:08 GMT
References: <1990Nov13.000139.15018@murdoch.acc.Virginia.EDU>
Organization: University of Maine System
Lines: 18

I too have found that the EcoRI sites one expects to find flanking a
cDNA insert are often non-functional.  This proved to be the norm and
not the exception in my last experiments involving gt10.  I was able
to subclone HindIII/BglII fragments into HindIII/BamHI cut vectors.
Good Luck!.
 
 
 
 
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*                          *                                        *
*  Dan Laflamme            *                                        *
*  Dept. of Biochemistry   *                                        *
*  University of Maine     *                                        *
*  Orono, Maine 04469      *                                        *
*  (207) 582-2818          *                                        *
*                          *                                        *
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