Path: utzoo!attcan!uunet!zephyr.ens.tek.com!uw-beaver!cornell!rochester!pt.cs.cmu.edu!o.gp.cs.cmu.edu!andrew.cmu.edu!ms5h+ From: ms5h+@andrew.cmu.edu (Marci Swede) Newsgroups: sci.bio Subject: Re: Karyotyping procedure Message-ID: Date: 10 Nov 90 23:13:33 GMT References: <1567@beguine.UUCP> Organization: Carnegie Mellon, Pittsburgh, PA Lines: 7 In-Reply-To: <1567@beguine.UUCP> When I was an undergrad we did a Karyotype in our genetics lab. I can get you the exact details, but for now, this is what i remember. We scraped the inner cheek with a tooth pick to obtain cells. These were placed on a slide and the chromotin was dyed (i can't recall the dye). The cells were then photographed and a standard karyotype was performed. I'll get you the full protocol soon. Where in Maine are you?