Path: utzoo!utgpu!watserv1!watmath!uunet!bionet!AARDVARK.UCS.UOKNOR.EDU!RTHRIFT From: RTHRIFT@AARDVARK.UCS.UOKNOR.EDU (Richard Thrift) Newsgroups: bionet.jobs Subject: Are we having fun yet? Message-ID: <9012010701.AA29414@genbank.bio.net> Date: 1 Dec 90 07:01:00 GMT Sender: daemon@genbank.bio.net Lines: 119 November 29, 1990 Dear People: I am currently a postdoc, looking for a position in BASIC BIOCHEMICAL RESEARCH, preferably with relatively little (or no) teaching involved, in academia or industry. I am hoping for something with a reasonable possibility of becoming more stable than the typical postdoctoral position. My main scientific interests are the processes involved in protein secretion and in plasma lipoprotein biosynthesis and secretion, but I am also interested in many other areas, including the structure, function, and metabolism of secretory and membrane proteins in general, lipid-protein interactions, and post- translational modifications. My thesis project involved the characterization of lipoproteins produced by a human liver cell line, to model plasma lipoproteins as they are secreted in vivo before being modified in the circulation. Subsequently I investigated the possibility that apolipoprotein B is a transmembrane protein at an early stage in the assembly of VLDL (Very Low Density Lipoprotein), and found evidence for a cytoplasmic mode of intracellular degradation of uncomplexed apo B. The research I am currently doing with Dr. Art Johnson (in collaboration with Dr. Peter Walter) is directed at the secretory pathway itself; specifically the identification of microsomal proteins involved in the integration of nascent membrane proteins into, and translocation of secretory proteins across, the endoplasmic reticulum membrane. Plasmids coding for membrane or secretory proteins are linearized at appropriate sites within the coding sequence. Transcription and translation produces truncated peptides of defined length which, due to the lack of proper termination, retain the tRNA and ribosomes at their COOH-termini. If the translations are done in the presence of microsomes the ribosomes prevent the C-terminus from being translocated across the E.R. membrane, thus trapping the peptide in transit across the membrane. Photolysis at this point causes the peptide to become crosslinked to specific ER proteins which are involved in the translocation process. This is an unusual approach, which may also be useful in studying other co- or post-translational interactions, including the formation of oligomeric complexes such as the T- cell antigen receptor, or viral assembly. I will be at the ASCB meeting in San Diego December 10-13, and have a poster there (#430) Monday. Please feel free to call, write, or E-mail. (By the way, from the notices I've seen here previously I don't really expect this notice to reach many potential employers. I am quite curious to see whether my impression is right) _______________________________________________________________________________ RICHARD N THRIFT Personal Data Addresses: University of Oklahoma 419 Forest Dr. Department of Chemistry and Biochemistry Norman, OK 73069 620 Parrington Oval (405) 360-7964 Norman, OK 73019-0370 (405) 325-3551 Internet: Rthrift@Aardvark.ucs.uoknor.edu Bitnet: Rthrift@Uokucsvx Social Security Number: 546-94-9252 Born: August 27, 1953, Owensboro, KY Marital status: Married; two children Education AB (Biophysics) 8/79 University of California at Berkeley MS (Biophysics) 6/82 University of California at Berkeley PhD (Biophysics) 12/86 University of California at Berkeley Primary Research Interests Protein and lipoprotein biosynthesis and secretion; mechanisms of protein translocation and targeting; protein structure/function; post-translational modifications; lipid-protein interactions. Recent Employment 9/88-present Postdoctoral Fellow; lab of Dr. Arthur E. Johnson, Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK. (Studying microsomal proteins involved in integration of nascent membrane proteins into the E.R.) 8/86-8/88 Postdoctoral Fellow; lab of Dr. Roger A. Davis, Hepatobiliary Research Center, University of Colorado Health Sciences Center, Denver, CO. (Studying assembly of apolipoprotein B into lipoproteins in rat liver.) 7/82-6/86 Graduate Student Research Associate/ PHS Predoctoral Trainee;lab of Dr. Trudy M. Forte, Biology and Medicine Division, Lawrence Berkeley Laboratory, University of California, Berkeley, CA. (Characterization of lipoprotein secretion by Hep G2 cells in culture.) 7/80-12/80 Research Assistant, lab of Dr Bradley J. Benson, Cardiovascular Research Institute, University of California, San Francisco, CA. (Analysis of pulmonary surfactant components.) Honors and Awards Colorado Heart Association Postdoctoral Research Fellowship, "Determination of Cytoplasmic Domains of Apolipoprotein B in Microsomes". $21,310. 7/1/87-6/30/88. Best student presentation, 11th Western Regional Meeting of Electron Microscopists, 5/83. Publications Thrift, RN, DW Andrews, P. Walter, and AE Johnson. Components of the translocon: The transmembrane segment of a nascent membrane protein is located adjacent to specific E.R. membrane proteins until termination of protein synthesis. Ms. submitted for publication. Davis, RA, RN Thrift, CC Wu, and KE Howell. 1990. Apolipoprotein B is both integrated into and translocated across the endoplasmic reticulum membrane: evidence for two functionally distinct pools. J. Biol. Chem. 265: 10005- 10011. Lee, LY, WA Mohler, BL Schafer, JS Freudenberger, N Byrne-Connolly, KB Eager, ST Mosly, JK Leighton, RN Thrift, RA Davis, and RD Tanaka. 1989. Nucleotide sequence of rat low density lipoprotein receptor cDNA. Nucleic Acids Res. 17: 1259-1260. Chen, C-H, TM Forte, BE Cahoon, RN Thrift, and JJ Albers. 1986. Synthesis and secretion of lecithin-cholesterol acyltransferase by the human hepatoma cell line HepG2. Biochim. Biophys. Acta 877: 433-439. Thrift, RN, TM Forte, BE Cahoon, and VG Shore. 1986. Characterization of lipoproteins produced by the human liver cell line, Hep G2, under defined conditions. J. Lipid Res. 27: 236-250. Thrift, RN, TM Forte, and RW Nordhausen. 1983. Low density lipoprotein receptor localization using a colloidal gold label and surface replication. Texas Society for Electron Microscopy Journal. 14(3): 25. Brought to you by Super Global Mega Corp .com