Path: utzoo!utgpu!watserv1!watmath!uunet!bionet!aberystwyth.ac.uk!DBK
From: DBK@aberystwyth.ac.uk
Newsgroups: bionet.molbio.methds-reagnts
Subject: Yeast protoplasts /lyticase
Message-ID: <9012030854.AA27781@genbank.bio.net>
Date: 3 Dec 90 08:54:51 GMT
Sender: daemon@genbank.bio.net
Lines: 10

Does anyone know a good enzyme (and source!) for degrading yeast
(Saccharomyces cerevisiae) cell walls to make protoplasts? We have
had a go with Sigma lyticase (mixture of stuff from Arthrobacter), which
certainly lives up to its name (i.e. clobbers the protoplasts after making
them). Once upon a time there was helkicase from snails, which is supposed to
be good (not in Sigma catalogue). [that was helicase!]

All help *VERY* gratefully received.
Many thanks,
Douglas Kell.


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