Path: utzoo!utgpu!watserv1!watmath!att!news.cs.indiana.edu!bionet!citiago.bitnet!arenas
From: arenas@citiago.bitnet (Jaime Arenas)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Yeast protoplasts /lyticase
Message-ID: <9012062122.AA06775@genbank.bio.net>
Date: 6 Dec 90 21:06:47 GMT
References: <901206023912.20800133@Iago.Caltech.Edu>
Sender: daemon@genbank.bio.net
Lines: 31

> Does anyone know a good enzyme (and source!) for degrading yeast
> (Saccharomyces cerevisiae) cell walls to make protoplasts? We have
> had a go with Sigma lyticase (mixture of stuff from Arthrobacter), which
> certainly lives up to its name (i.e. clobbers the protoplasts after making
> them). Once upon a time there was helkicase from snails, which is supposed to
> be good (not in Sigma catalogue). [that was helicase!]


We always use Zymolyase from  SEIKAGAKU with good results.

ZYMOLYASE - 100T
CAT# 120493
SEIKAGAKU AMERICA INC.
P.O. BOX 21517
St. Petersburg, FL 33742

info   (800) 237-4512
telex  52-9422 SKK STB
Fax    (813) 576-1870


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Jaime Arenas, Ph.D.
Division of Biology 147-75
CALTECH
Pasadena, CA 91125

ARENAS@CITIAGO.BITNET
ARENAS@IAGO.CALTECH.EDU



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