Path: utzoo!utgpu!watserv1!watmath!uunet!bionet!LANL.GOV!pgil%histone
From: pgil%histone@LANL.GOV (Paul Gilna)
Newsgroups: bionet.molbio.genbank
Subject: Re:  lambda gt11 ?
Message-ID: <9012071849.AA00831@histone.lanl.gov>
Date: 7 Dec 90 18:49:45 GMT
Sender: daemon@genbank.bio.net
Lines: 20


I may well stand to be corrected on this but.....


 To the best of our knowledge, the complete sequence of gt11 has never
 been published. The vector was basically constructed by tacking
 various expression and promotor sequences to a variant of lambda
 gt10, which itself was a construct, etc, etc,

 Traditionally GenBank has not reflected construct sequences unless
 those sequences were sequenced to confirm the construction: while this
 is easily done for plasmid sequences, it is unlikley that this would
 normally be done for lambda as it is rather large; if I remember from
 my wet lab days, gt11 is about 9kb.

 It is possible that VecBase at MIT has it.

Cheers,

Paul Gilna, GenBank, Los Alamos