Path: utzoo!utgpu!watserv1!watmath!uunet!cs.utexas.edu!uwm.edu!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Synthetic E.coli synthetic transcription-repair substrate. Message-ID: Date: 12 Jan 91 13:01:59 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 28 Approved: lear@genbank.bio.net Checksum: 29688 2 LOCUS ECOTAC 137 bp ds-DNA SYN 12-JAN-1991 DEFINITION Synthetic E.coli synthetic transcription-repair substrate. ACCESSION M57247 J05700 KEYWORDS tac promoter; thymine-thymine dimer. SOURCE Synthetic E.coli DNA. ORGANISM Artificial gene Artificial sequences; Genes. REFERENCE 1 (bases 1 to 137) AUTHORS Selby,C.P. and Sancar,A. TITLE Transcription perferentially inhibits nucleotide excision repair of the template DNA strand in vitro JOURNAL J. Biol. Chem. 265, 21330-21336 (1990) STANDARD full staff_review FEATURES Location/Qualifiers -35_signal 12..16 /note="tac promoter" -10_signal 35..39 /note="tac promoter" misc_feature 48..49 /note="transcription start sites" misc_feature 74..75 /note="thymine-thymine cyclobutane dimer" BASE COUNT 35 a 34 c 27 g 41 t ORIGIN 1 cccatccccc tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gcagactcag 61 tacgaattcc tccaacttgc tgaggccatc gataaaggtc tagatctccc tatagtgagt 121 cgtattaatt tcgacgt //