Path: utzoo!utgpu!watserv1!watmath!att!pacbell.com!ucsd!usc!zaphod.mps.ohio-state.edu!rpi!dali.cs.montana.edu!milton!dadler
From: dadler@milton.u.washington.edu (David Adler)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Anyone done PCR directly from cells
Message-ID: <14018@milton.u.washington.edu>
Date: 8 Jan 91 23:04:58 GMT
References: <1991Jan8.232000.1@csc.fi>
Organization: University of Washington, Seattle
Lines: 15


	Raimo Pollanen writes;
	Has anyone made PCR from tissue or cell samples without 
	extracting DNA by phenol-chlorophorm precipitations
	...

I have done a few PCR's on remnants of amniotic fluid
samples and it seemed to work fine.  Of course you want
to make sure cells are lysed - freezing and thawing
thru several cycles should work - this is in fact what
happened to amniotic fluid before I got it. I do not 
know how many cells but it was fractions of an original
1 ml of amniotic fluid, so not many.  I amplified
a repetitive target, maybe 1000 copies per cell, as well
as a single copy sequence.