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From: WEH%FDACFSAN@PUCC.PRINCETON.EDU
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Anyone done PCR directly from cells
Message-ID: <9101101327.AA08335@genbank.bio.net>
Date: 10 Jan 91 12:36:41 GMT
Sender: daemon@genbank.bio.net
Lines: 5

We've had good success with some bacterial cells by simply boiling for
a minute or two.  Depending on how you want to do it, a couple of
microliters of this *boilate* can be added directly to a PCR tube or you
can try precipitating with ethanol.  You can also lyse cells with
guanidine isothiocyanate and then ethanol precipitate.