Path: utzoo!utgpu!news-server.csri.toronto.edu!bonnie.concordia.ca!uunet!samsung!sol.ctr.columbia.edu!cica!bronze!cricket.bio.indiana.edu!gilbertd From: gilbertd@cricket.bio.indiana.edu (Don Gilbert) Newsgroups: bionet.molbio.methds-reagnts Subject: Re: Further info on Help needed with a plasmid Message-ID: <1991Jan16.150308.15678@bronze.ucs.indiana.edu> Date: 16 Jan 91 15:03:08 GMT References: <247.27934eb5@mbcl.rutgers.edu> Sender: news@bronze.ucs.indiana.edu (USENET News System) Organization: Biology, Indiana University - Bloomington Lines: 22 In article <247.27934eb5@mbcl.rutgers.edu> friedman@mbcl.rutgers.edu writes: > >I was didn't notice that after the pasting of the text into the news group, the >plasmid name was left off. The plasmid is pW8. It is apparently a P-element >vector used for inserting DNA into the genome of Drosophila melanogaster. If this is any help, there is a PW8(something) mentioned in John Merriam's Drosophila clone list. Here are the entries. clonelist.txt:7D1,2 [PW8hsp-sev ch41]hsp,sev 3 0 Science243:931 clonelist.txt:46E [PW8hsp-sev ch21]hsp,sev 3 0 Science243:931 clonelist.txt:93B [PW8hsp-sev ch50]hsp,sev 3 0 Science243:931 The first column is cytological map location, the next [PW8...] is gene name the third ("3") is "transformed inserts", the fourth column ("0") is size in kilobases, and the last is a reference. This clonelist is available via anonymous ftp from iubio.bio.indiana.edu, directory [archive.fly] -- Don -- Don Gilbert gilbertd@cricket.bio.indiana.edu biocomputing office, biology dept., indiana univ., bloomington, in 47405