Path: utzoo!utgpu!watserv1!watmath!uunet!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: E.coli phosphoenolpyruvate carboxykinase (pckA) gene, complete cds. Message-ID: Date: 31 Jan 91 13:13:40 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 70 Approved: lear@genbank.bio.net Checksum: 04299 4 LOCUS ECOPCKA 1860 bp ds-DNA BCT 31-JAN-1991 DEFINITION E.coli phosphoenolpyruvate carboxykinase (pckA) gene, complete cds. ACCESSION M59823 M34605 KEYWORDS pckA gene; phosphoenolpyruvate carboxykinase. SOURCE E.coli (K12) DNA. ORGANISM Escherichia coli Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively anaerobic rods; Enterobacteriaceae. REFERENCE 1 (bases 1 to 1860) AUTHORS Medina,V., Pontarollo,R., Glaeske,D., Tabel,H. and Goldie,H. TITLE Sequence of the pckA gene of Escherichia coli K-12: Relevance to genetic and allosteric regulation and homology of E.coli phosphoenolpyruvate carboxykinase with the enzymes from Trypanosoma brucei and Saccharomyces cerevisiae JOURNAL J. Bacteriol. 172, 7151-7156 (1990) STANDARD simple staff_entry FEATURES Location/Qualifiers -35_signal 101..106 /gene="pckA" -10_signal 127..133 /gene="pckA" mRNA 140..1673 /gene="pckA" /partial /product="phosphoenolpyruvate carboxykinase" misc_binding 247..254 /note="nucleotide binding site" RBS 267..271 /gene="pckA" CDS 279..1673 /EC_number="4.1.1.49" /gene="pckA" /codon_start=279 /product="phosphoenolpyruvate carboxykinase" misc_binding 316..328 /note="calcium binding site" BASE COUNT 476 a 474 c 455 g 455 t ORIGIN 1 atcgatactt taccggttga atttgcattt tcattcagga atgcgattcc actcacaata 61 ttcccgccat ataaaccaag atttaacctt ttgagaacat tttccacacc taaaatgcta 121 tttctgcgat aatagcaacc gtttcgtgac aggaatcacg gagttttttg tcaaatatga 181 atttctccag atacgtaaat ctatgagcgt tgtcgcggtt aacaccccca aaaagacttt 241 actattcagg caatacatat tggctaagga gcagtgaaat gcgcgttaac aatggtttga 301 ccccgcaaga actcgaggct tatggtatca gtgacgtaca tgatatcgtt tacaacccaa 361 gctacgacct gctgtatcag gaagagctcg atccgagcct gacaggttat gagcgcgggg 421 tgttaactaa tctgggtgcc gttgccgtcg ataccgggat cttcaccggt cgttcacaaa 481 agataagtat atccgtccgt gacgatacca ctcgcgatac tttctggtgg gcagacaaag 541 gcaaaggtaa gaacgacaac aaacctctct ctccggaaac ctggcagcat ctgaaaggcc 601 tggtgaccag gcagctttcc ggcaaacgtc tgttcgttgt cgacgctttc tgtggtgcga 661 acccggatac tcgtctttcc gtccgtttca tcaccgaagt ggcctggcag gcgcattttg 721 tcaaaaacat gtttattcgc ccgagcgatg aagaactggc aggtttcaaa ccagacttta 781 tcgttatgaa cggcgcgaag tgcactaacc cgcagtggaa agaacagggt ctcaactccg 841 aaaacttcgt gcgtttaacc gaccgagcgc atgcagctga ttgcggcacc tggtacggcg 901 gcgaaatgaa gaaagggatg ttctcgatga tgaactacct gctgccgctg aaaggtatcg 961 cttctatgca ctcgtccgcc aacgttggtg agaaaggcga tgttgcggtg ttcttcggcc 1021 tttccggcaa cggtaaaaca gcctttccac gaccgaaacg tcgcctgatt ggcgatgacg 1081 aacacggctg ggacgatgac ggcgtgttta acttgaaggc ggctgggtac gcaaaaacta 1141 tcaagctgtc gaaagaagcg gaacctgaaa tctacaagct atccgtcgtg atggcgttgc 1201 tggaaaacgt caccgtgcgt gaagatggca ctatcgactt tgatgatggt tcaaaaaccg 1261 agaacacccg cgtttcttat ccgatctatc acatcgataa cattgttagg ccggtttcca 1321 aagcgggcca cggacgaagg ttatcattgc tgactgctca tgctttcggc gtgttgccgc 1381 cggtttctcg cctgactgcc gatcaaaccc agtatcactt cctctctggc ttcaccgcga 1441 aactggccgg tactgagcgt ggcatcaccg aaccgacgcc aaccttctcc gcttgcttcg 1501 gcgcggcatt cctgtcgctg cacccgactc agtacgcaga agtgctggtg aaacgtatgc 1561 aggcggcggg cgcgcaggct tatctggtta acactggctg gaacggcagt ggcaaaagta 1621 tctcgattaa agataccgcg cattatcgac gcatctcacg gttcgctgga taatcgaaac 1681 tttaactgtt acctgcatcg caatcgaatc cgggcgtaga agcgatcaat tctcgatccg 1741 cgtaacacta cgcttatcgg tcatgcagaa acgaactgcg aactctttat cgaattgtag 1801 taacgaacct cgtcgtcgta cctgtcaatt atatcatcga atatcaacta gcagttaagg //