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From: bwoodman@magnus.ircc.ohio-state.edu (Robert H Woodman)
Newsgroups: bionet.general,bionet.molbio.methds-reagnts
Subject: Need a _Streptomyces_ plasmid
Summary: A beta-lactamase expressible in _Streptomyces_ needed.
Keywords: _Streptomyces_, beta-lactamase, _E. coli_
Message-ID: <1991Feb15.181951.7065@magnus.ircc.ohio-state.edu>
Date: 15 Feb 91 18:19:51 GMT
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Originator: bwoodman@left.magnus.ircc.ohio-state.edu
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	Hello, fellow bionetters!  I have for a considerable period of time 
desired to have a particular kind of plasmid for use with the genus 
_Streptomyces_.  The plasmid I have in mind is an _E. coli_/_Streptomyces_ 
shuttle vector.  The plasmid should *not* contain the resistance gene for 
thiostrepton/nosiheptide.  The resistance gene I wish to see on the plasmid is 
a penicillin/ampicillin resistance (beta-lactamase) gene.  The gene should 
either be expressible in both _Streptomyces_ and _E. coli_ (as you may know, 
_E. coli_ can use some _Streptomyces_ promotors), or there should be two copies
of the gene -- one each for expression in the two different organisms.  Finally,
the plasmid should have a useful polylinker or at least several useful cloning 
sites.
	
	If anybody has such a plasmid (or even just a beta-lactamase expressible
in _Streptomyces_), and if you can share it, would you please let me know?  I'd
like to work with such a plasmid in _Streptomyces actuosus_, the nosiheptide 
producer.  (Note:  I'm not too lazy to invent such a plasmid if one doesn't
exist, but I thought that before I tackled such a project, I'd make sure that
I wasn't re-inventing the wheel.)

	
	Thanks for any help offered.
	
	Robert H. Woodman
	
	
-- 
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*Bob Woodman                  * "A job not worth doing well is not  *
*INTERNET:  woodman.1@osu.edu *  worth doing."--Salvador Luria      *
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