Path: utzoo!utgpu!watserv1!watmath!uunet!bionet!GENBANK.BIO.NET!kristoff From: kristoff@GENBANK.BIO.NET (Dave Kristofferson) Newsgroups: bionet.molbio.genbank Subject: Re: message Message-ID: Date: 14 Feb 91 18:24:43 GMT Sender: kristoff@genbank.bio.net Lines: 149 > > I a medical student at the University of Arizona and I am doing research > involving gene amplification of the human collagenase IV gene. Could you > please send me information concerning your Human GENE Database(i.e. is there > some way I can get connect to the GENEBANK database so that I can search for > my gene sequence? Is there a manual I might need? > > Thanks for your time and help, > John Pierpont > > To obtain instructions for doing GenBank searches by e-mail send a message containing the word HELP to search@genbank.bio.net I include information about the GenBank On-line Service below which allows you more extensive access to the database. Sincerely, Dave Kristofferson GenBank Manager kristoff@genbank.bio.net ---------------------------------------------------------------------- GenBank On-line Service Features ------------------------------------ **** New Solbourne Computer A powerful 80 MIPS (million instructions per second) Solbourne superminicomputer is used for all aspects of the GenBank On-line service. A 1000 base query sequence can be compared against all of GenBank in about 12 minutes using the FASTA similarity searching program (see below). **** Daily Updates of the GenBank, GenPept, and EMBL Databases Users can now retrieve and search the latest GenBank and EMBL sequence entries. A database (GenPept) containing translations of all annotated open reading frames in GenBank is also available. "New" databases of recent GenBank, GenPept, and EMBL entries are updated daily with data supplied by Los Alamos National Laboratory and the EMBL. Both the IRX and FASTA programs search these new entries. **** Fast, Indexed, Annotation Search and Entry Retrieval The IRX software from the National Library of Medicine is used to provide fast retrieval of database entries by any combination of words within the entry annotations. Sequences identified by the program may then be downloaded using the Kermit protocol or saved to the user's directory. **** Similarity Searching Using FASTA All of the above databases may be searched using the FASTA program written by William Pearson. The TFASTA program which compares protein sequences to the nucleic acid databases in up to 6 reading frames by translating at runtime is also available. **** Electronic Communications and Bulletin Boards (Newsgroups) Users with accounts on the GenBank On-line Service computer can use its electronic mail facilities to communicate at no cost with colleagues worldwide. Access to the numerous USENET newsgroups is available. Questions on a wide variety of subjects can be posted for an international audience. Of primary interest for the GenBank community are the BIOSCI newsgroups which cover subjects such as laboratory methods, journal contents, protein analysis, NIH funding announcements, and biological software. **** Access to the IntelliGenetics Suite of Programs For an additional fee, Class II users will also have access to all of the IntelliGenetics Suite of molecular biology sequence analysis software and the databases which are distributed with that software. This software provides a comprehensive range of functionality and is described in a separate enclosure. **** Connection by Public Data Network or by Internet The GenBank On-line Service computer can be accessed by the SprintNet Public Data Network using a modem and phone lines or, for users that have an account on a computer with access to the Internet, by interactive connection using the TELNET protocol. User Class Descriptions ----------------------- Two different classes of login accounts exist on the GenBank computer and are referred to as Class I and Class II accounts as described below. In addition certain services are provided at no charge for the community at large through either anonymous login to the GenBank computer or by electronic mail server. Both non-profit AND for-profit users may access the GenBank On-line Service. The same fee structure applies to both types of users (contact us as indicated below for details). >>>> Services available to Class I (basic) time-sharing accounts ** Private login account with 1 Mbyte disk space. ** Daily database updates. ** Unrestricted access to databases through the IRX program. ** Interactive and batch use of FASTA and TFASTA similarity search programs. ** Access to worldwide electronic mail communication and all USENET newsgroups including the 18 BIOSCI (bionet.*) newsgroups specializing in topics for molecular biologists. >>>> Services available to Class II time-sharing accounts ** All of the Class I services. ** Access to all IntelliGenetics Suite programs and databases. >>>> Services available at no cost **Limited 20 min. IRX sessions to download database entries.** To use this anonymous access, dial 415-961-6860 for 1200 or 2400 baud access (415-961-6864 for 9600 baud Telebit Trailblazer access). Then use the GenBank username "genbank" with the password "4nigms" to log into the public account. You will be placed into the IRX program automatically. Access is also possible by the Internet. "TELNET" to the computer "genbank.bio.net" and log in with the GenBank username and password given above. **FASTA similarity searching by electronic mail.** To get instructions for using the FASTA Server, send an e-mail message with the command "help" in the body of the message to the Internet address "search@genbank.bio.net". **Sequence retrieval by locus name or accession number through an electronic mail server.** Instructions are available by sending a message similar to the above to the address retrieve@genbank.bio.net. **Daily/Weekly updates of GenBank, GenPept, and EMBL by anonymous FTP.** For further information please call GenBank at 415-962-7364, send e-mail to gos@genbank.bio.net, or write us at GenBank On-line Service c/o IntelliGenetics, Inc. 700 E. El Camino Real Mountain View, CA 94040 U.S.A.