Path: utzoo!censor!comspec!humvax!becker!ncrcan!attcan!uunet!zaphod.mps.ohio-state.edu!usc!apple!snorkelwacker.mit.edu!bloom-beacon!eru!hagbard!sunic!fuug!funic!csc.fi!pollanen From: pollanen@csc.fi Newsgroups: bionet.molbio.methds-reagnts Subject: Help me in in vitro translations Message-ID: <1991Jan7.151654.1@csc.fi> Date: 7 Jan 91 15:16:54 GMT Sender: usenet@nic.funet.fi Lines: 13 Hello Has anyone made in vitro translations with reticulo lysate and purified mRNA preparates? Amershams Tobacco Mosaic Virus reference works well but my own mRNA not I am using TMV enzyme reverse transcriptase from AMV and normal in vitro translation protocols... But it does not work Please help me if you can ! Sincerely Raimo Pollanen (M.Sc.) E-Mail Pollanen@CSC.FI