Path: utzoo!utgpu!news-server.csri.toronto.edu!bonnie.concordia.ca!ccu.umanitoba.ca!frist
From: frist@ccu.umanitoba.ca
Newsgroups: bionet.molbio.methds-reagnts
Subject: ELISA for DNA/RNA?
Message-ID: <1991Feb19.223845.25660@ccu.umanitoba.ca>
Date: 19 Feb 91 22:38:45 GMT
Organization: University of Manitoba, Winnipeg, Canada
Lines: 26

Has anybody come up with a way of fixing DNA or RNA to the bottoms of
microtiter plate wells? Say, for example you wanted to fix total RNA
samples from a large number of tissue samples (eg. a timecourse
experiment), and then hybridize with a biotinylated probe. Next add
streptavidin-conjugated alkaline phosphatase, and substrate, and read the
result in your microtiter plate reader.  Essentially  we're talking about
an ELISA for DNA.

---------|           |-----------
         |   %%%%%   | streptavidin-conjugated enzyme
          \  ----   / biotinylated antisense RNA probe
           \ ~~~~  /  unlabeled total RNA (fixed to plate)
            ------     

This is such an obvious idea that I would think there must be a kit
somewhere for it, but I don't recall having seen it in the literature.

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Brian Fristensky                | What can literature do against the pitiless 
Department of Plant Science     | onslaught of naked violence? Let us not for-
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FAX:            204-275-5128    |     Alexander Solzhenitsyn, NOBEL LECTURE 
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