Path: utzoo!utgpu!watserv1!watmath!uunet!wuarchive!zaphod.mps.ohio-state.edu!caen!news.cs.indiana.edu!ariel.unm.edu!KIM@m44.unm.edu
From: kim@m44.unm.edu
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: ELISA for DNA/RNA?
Message-ID: <0094482B.70D0C1E0@m44.unm.edu>
Date: 20 Feb 91 18:06:36 GMT
References: <1991Feb19.223845.25660@ccu.umanitoba.ca>
Reply-To: kim@m44.unm.edu
Organization: UNM Cancer Center, Albuquerque, NM
Lines: 11

The kind of assay you described seems to be functionally similar to a dot
blot/slot blot, except that slots cannot be read by a microplate reader.
A really indirect sandwich that may work would be to allow avidin to adhere
to the bottoms of the wells, and then use biotinylated total RNA (Maybe 
photobiotinylated).  Or, to be even more indirect, fix avidin to the plate,
then put biotinylated oligo-dT into the well for the second layer of the 
sandwich.  Wash the well, then apply your total RNA.  Poly(A) RNA will then
hybridize with the oligo(dT).  Do another wash, not disrupting the hybrids,
and then add your probe.  

Daniel Kim