Path: utzoo!utgpu!watserv1!watmath!uunet!bionet!MAINE.MAINE.EDU!KEITHH
From: KEITHH@MAINE.MAINE.EDU (Keith Hutchison)
Newsgroups: bionet.molbio.methds-reagnts
Subject: ELISA-based assay for DNA/RNA
Message-ID: <KEITHH.910220161732@maine.maine.EDU>
Date: 20 Feb 91 21:17:32 GMT
Sender: daemon@genbank.bio.net
Lines: 8

The sandwich method for detecting hybrids in an ELISA assay apparently
works very well as it is the basis of an assay from Hood's group
(see PNAS 87: 8923-8927 [1990]).  You would coat the wells with
streptavidin and biotinylate the RNA.  Then used digoxigenin to
tag the probe.  We haven't tried it yet, but it sounds pretty
straightforward.

Keith Hutchison