Path: utzoo!utgpu!news-server.csri.toronto.edu!bonnie.concordia.ca!uunet!bionet!SCRIPPS.EDU!collet
From: collet@SCRIPPS.EDU (Thomas Collet)
Newsgroups: bionet.general
Subject: restriction enzyme sites in mammalian cell
Message-ID: <9102222338.AA13207@terrapin.scripps.edu>
Date: 22 Feb 91 23:38:26 GMT
Sender: daemon@genbank.bio.net
Lines: 9

I am trying to clone immunoglobulins from hybridoma cells and
am wondering whether I am using the best possible restriction
enzymes.  Does anyone have a compilation of the cutting
frequencies of restriction enzymes in immunoglobulin genes (or
maybe just mammalian genes in general ?) Or maybe a literature
reference ?
Thanks

Thomas


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